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Old 11/29/2016, 01:29 PM   #326
jason2459
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Still had some "extras" even after all the rinsing but those strands I am pretty darn sure are from the vibrant.

Algae_vs_Vibrant2-0006 by Jason, on Flickr

Algae_vs_Vibrant2-0007 by Jason, on Flickr


Algae_vs_Vibrant2-0008 by Jason, on Flickr

Algae_vs_Vibrant2-0009 by Jason, on Flickr


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Old 11/29/2016, 01:41 PM   #327
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Now this set was cool to watch and have it on video (hopefully) The pieces being "pulled" out of the algae.


Algae_vs_Vibrant2-0011 by Jason, on Flickr

Algae_vs_Vibrant2-0018 by Jason, on Flickr

Algae_vs_Vibrant2-0019 by Jason, on Flickr

Algae_vs_Vibrant2-0020 by Jason, on Flickr

Algae_vs_Vibrant2-0021 by Jason, on Flickr

Algae_vs_Vibrant2-0022 by Jason, on Flickr

Algae_vs_Vibrant2-0023 by Jason, on Flickr

Algae_vs_Vibrant2-0024 by Jason, on Flickr

Algae_vs_Vibrant2-0025 by Jason, on Flickr

Algae_vs_Vibrant2-0026 by Jason, on Flickr


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Old 11/29/2016, 07:09 PM   #328
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Google decided to animate that sequence. I still need to get the videos uploaded.




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Old 11/29/2016, 10:06 PM   #329
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Playlist
Algae with Vibrant
https://www.youtube.com/playlist?lis...D7gZ9OkfQYvwdT


And this was the cool one.

Right about the 2 minute mark I try to see where these little pieces are coming from. Then realized they were coming from the algae...


Right after the 3 minute mark I caught a nice chuck come out.





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Old 11/30/2016, 01:59 AM   #330
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Definitely an interesting result


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Old 12/03/2016, 09:16 PM   #331
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Quote:
Originally Posted by jason2459 View Post
Back to Bacteria in a bottle. I've looked at Instant Ocean Biospira, Dr. Tim's one and only, and Dr. Tim's Eco Balance.

Of those it was only the one and only I didn't find any bacteria but plenty of detritus.

Tonight I'm looking at Microbacter7. It reminds me a lot of the Eco Balance but there is visually some differences in some of the bacteria.





I'm going to try this product on my reef, I have a little cyano, and very small patches of hair algae. I keep my nutrients pretty low, barely detectable if at all. I want to know if these products help with the nitrogen processing by improving the bacteria diversity. We shall see.


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Old 12/04/2016, 06:18 AM   #332
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Until now trimming my ATS and chaeto was a sad moment, as I knew that I threw away, a lot of useful organisms. Seeing you photos make it a lot worse.


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Old 12/08/2016, 11:03 AM   #333
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I'm interested in the Vibrant test. Btw your work here is interesting and important!


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Old 12/08/2016, 12:06 PM   #334
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Thanks. I don't know how important it really is but it is interesting.


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Old 12/13/2016, 12:02 PM   #335
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I think I've narrowed down what this smaller dinoflagellate is. I've suspected a type of Amphidinium.






From the description in this paper I'm thinking it's Amphidinium massartii. Some really cool images in this article too.
https://www.researchgate.net/publica...u_Island_Korea

From it:
Amphidinium massartii Biecheler (1952) and Amphi-
dinium carterae Hulburt (1957) are small and have some-
times been considered to be con-specific (Murray et al.
2004, 2012). However, Murray et al. (2004) suggested that
A. massartii is clearly different from A. carterae because
A. massartii plastids are centrally located and are pres-
ent at a relatively low density, while A. carterae plastids
are peripherally located and reticulated. In addition, they
also suggested that flagellar insertion of A. massartii (~0.6
of the cell body from the apex) is situated in a lower po-
sition than in A. carterae (~0.4 of the cell body from the
apex).

From their toxicity testing:
Toxicity analysis
No nauplii of A. salina were dead after 48 h of incuba-
tion with A. massartii AMJJ1 concentrations of 100, 500,
1,000, 5,000, or 10,000 cells mL


Another good article with images of various dinoflagellate around Jeju island as well.
http://www.kpubs.org/article/article...2013_v36n4_347


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Old 01/11/2017, 02:04 AM   #336
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I have what looks like a dusty dark dirt like area on my substrate where my last bit of cyano was during my Vibrant testing and I wanted to see what all was there.







Pics and vids uploading. I will give a little spoiler because I was very excited to find them. I finally found a couple ostracods with eggs. Very cool looking.


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Old 01/11/2017, 09:24 AM   #337
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So, it mostly looked like well... dirt. It was a lot of detritus with some cyano, diatoms, sponge spicules, lots of bacteria of different types etc. Normal detritus stuff I see.

But I did find some cool stuff.

I followed this little ciliate for quite some time. It was very excited spinning very fast.
Dirt_on_Sand-0013 by Jason, on Flickr


Some cyano but not a lot
Dirt_on_Sand-0015 by Jason, on Flickr

A few Diatoms
Dirt_on_Sand-0017 by Jason, on Flickr

Dirt_on_Sand-0035 by Jason, on Flickr


Some walking ciliates
Dirt_on_Sand-0019 by Jason, on Flickr

Dirt_on_Sand-0005 by Jason, on Flickr

Sponge Spicules and here's a tight ball of them
Dirt_on_Sand-0034 by Jason, on Flickr


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Old 01/11/2017, 10:08 AM   #338
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And lots of ostracods

Dirt_on_Sand-0001 by Jason, on Flickr

Dirt_on_Sand-0008 by Jason, on Flickr

Dirt_on_Sand-0018 by Jason, on Flickr

and found some that were hold eggs. This was very cool to see and see some of various sizes.
Dirt_on_Sand-0006 by Jason, on Flickr

Dirt_on_Sand-0031 by Jason, on Flickr

Dirt_on_Sand-0007 by Jason, on Flickr

Dirt_on_Sand-0010 by Jason, on Flickr

Dirt_on_Sand-0025 by Jason, on Flickr

Dirt_on_Sand-0028 by Jason, on Flickr

Dirt_on_Sand-0029 by Jason, on Flickr


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Old 01/11/2017, 10:39 AM   #339
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Old 01/23/2017, 08:31 PM   #340
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This should be pretty cool





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Old 01/23/2017, 09:38 PM   #341
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Wow, amazing post. I forgot how much fun it is to look through a microscope. Plus the camera allows capture. Very cool! Thank you for posting.


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Old 01/24/2017, 08:46 AM   #342
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I found the 4 they show on the label and then some. A nice bacteria count as well.

Ocean_Magik_Phytoplankton-0001 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0002 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0003 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0004 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0005 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0006 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0008 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0014 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0016 by Jason, on Flickr


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Old 01/24/2017, 05:13 PM   #343
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Interesting. It'd be very interesting to find a way to estimate the volume of phytoplankton a a percentage, but that probably difficult, to say the least.


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Old 02/07/2017, 05:37 AM   #344
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Hey, Jason. Have you ever run across these before?
I've seen them a couple of times but no idea what they are.
super tiny (cyano for size comparison), swim with a spiraling motion - see video 1. Pigmentation like dinoflagellate. Shaped flat like a flea.
vid 1
https://youtu.be/qFZ_Xrjo1HY

higher power vid 2
https://youtu.be/DR0qZlgSgc8

Live with cyano on sandbed - see pic


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Old 02/07/2017, 06:27 AM   #345
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Quote:
Originally Posted by taricha View Post
Hey, Jason. Have you ever run across these before?
I've seen them a couple of times but no idea what they are.
super tiny (cyano for size comparison), swim with a spiraling motion - see video 1. Pigmentation like dinoflagellate. Shaped flat like a flea.
vid 1
]

Live with cyano on sandbed - see pic
Yes, dinoflagellets for sure. Possibly a type of Amphidinium


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Old 02/07/2017, 09:14 PM   #346
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Thanks for reply and awesome thread. I got lucky and found an ID I think I like - (movement doesn't seem a match for amphidinium dino).
Cryptomonas
and maybe more specifically a Rhodomonas
I got a better detailed video
https://youtu.be/IP0znkYW9xw
It's cool. It's a whole different class of thing than what I've ID'd in my tank before.


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Old 02/07/2017, 09:38 PM   #347
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what microscope are you using to get those videos? I can't see anything that good with mine.


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Old 02/07/2017, 10:12 PM   #348
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Quote:
Originally Posted by karimwassef View Post
what microscope are you using to get those videos? I can't see anything that good with mine.
heh, it's a sad old high school scope (30 yr old prolly) maybe this one that was discarded by biology class - shot through galaxy 4 phone.


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Old 02/07/2017, 10:30 PM   #349
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Quote:
Originally Posted by taricha View Post
Thanks for reply and awesome thread. I got lucky and found an ID I think I like - (movement doesn't seem a match for amphidinium dino).
Cryptomonas
and maybe more specifically a Rhodomonas
I got a better detailed video
https://youtu.be/IP0znkYW9xw
It's cool. It's a whole different class of thing than what I've ID'd in my tank before.
Nice. What could help is if you could close the iris to help increase the contrast and see if you can spot two flagella.

Easier if you can capture with vid that could increase exposure and boost contrast as well. And even then it could be hard.

Looking it up even the first ID sketch of that species only had a single flagellet. Also looks like it may be one of the non-toxic species. So they would be a score minus when they bloom. lol


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Old 02/07/2017, 10:59 PM   #350
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Quote:
Originally Posted by jason2459 View Post
Nice. What could help is if you could close the iris to help increase the contrast and see if you can spot two flagella.
Still shot of what looks like flagella coming out of the grooved end (or maybe my imagination)


And here's a few still shots stitched into animation





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